Mycotoxin Detector In Food Feed Grease Dairy Products Medicine Beverage Wine ST2000A

Mycotoxin Detector in food, food, feed, grease, dairy products, medicine, beverage, wine

ST-2000A Mycotoxin Detector

ST-2000A Mycotoxin analyzer is a necessary analytical instrument for the analysis of flavotoxin and enzyme-linked immunoassay.

Using the principle of solid phase enzyme-linked immunosorbent ELISA, namely enzyme-linked immunosorbent assay, which is composed of this instrument and B1 kit, can limit and quantitatively determine the content of aflatoxin B1 in the sample (if matched with other kits, other toxins can be measured).

It is widely used in the detection of aflatoxin B1 and other mycotoxins in food, food, feed, grease, dairy products, medicine, beverage, wine and other products.

Performance characteristics:

1, display operation: color LCD screen, visual voice cloth board, display the whole board information, touch screen operation

2, vibration plate function: 3 stage vibration plate speed, time 0-255 seconds adjustable

3, Work station: professional software of enzyme plate analyzer, can store 500 groups of programs, 100000 sample results, provide absorbance, linear equation, logarithmic equation, quadratic equation, cubic equation, absorbance percent-concentration logarithmic equation, spline function, inhibition rate and other rich calculation models

Technical parameters:

Light source: DC12V 22W imported halogen lamp

Wavelength range: 400-900nm

Filter: standard 405, 450, 492, 630nm, other wavelength optional

Reading range: 0-4.000Abs

Discriminating rate: 0.001 ABS

Indicated value error: ≤± 0.01abs

Stability: ≤±0.003Abs

Reproducibility: ≤0.3%

Size: 400mm(L)*260mm(W)*200mm(H) Weight: 12kg

Principle and application

This kit is used to detect Aflatoxin B1 (AFB1) in grain and feed samples by indirect competitive ELISA method. The kit consists of precoated antigen conjugated plate, horseradish enzyme marker, antibody, standard substance and other supporting reagents.

Test, to join the standard and sample solution, aflatoxin B1 and enzyme label plate sample pre package is coupling antigen antibody of aflatoxin B1, competition after joining enzyme markers, with TMB substrate color, sample absorbance value and content of aflatoxin B1 contained negative correlation, compared with the standard curve can be concluded that the residues of aflatoxin B1 in the sample.

2 Technical Indicators

2.1 Kit sensitivity: 0.03ppb(ng/ml)

2.2 Reaction mode: 25℃, 30min ~ 15min

2.3 Detection limit:

Grain.....................................................................0.15 PPB

Compound feed ………………………………0.3 PPB

Edible oil, peanuts …………………………0.6 PPB

Sauce class, wheat class and other feed ……………………0.3 PPB

Beer.....................................................................0.3 PPB

Wine, soy sauce, vinegar ……………………0.15 PPB

2.4 Cross reaction rate:

Aflatoxin B1…………………………100%

2.5 Sample recovery rate:

Corn and compound feed ……………………85%±15%

Peanut..................................................................82±15%

Cooking oil...............................................................85±15%

Sauce class, wheat class and other feed ………………83±15%

Beer...............................................................84±15%

Wine, soy sauce, vinegar ………………87±15%

3 Kit Composition

Enzyme label plate...................................................96 hole

Standard solution (black cap) : 1ml each

0ppb, 0.03ppb, 0.06ppb, 0.12ppb, 0.24ppb, 0.48ppb

High standard liquid: 100ppb…………………1ml

Enzyme marker (red lid) …………………5.5 ml

Antibody working fluid (blue cap) ………………5.5 ml

Substrate solution A (white cap) ……………………6ml

Substrate solution B (black cap) ……………………6ml

Termination solution (yellow cap) ………………………6ml

20X concentrated washing solution (white cap) ……………40ml

Manual...............................................................1

4 Required equipment and reagents

4.1 Instruments: aflatoxin tester, printer, homogenizer, nitrogen blow-dry device, oscillator, centrifuge, graduated pipet, balance (sensitivity 0.01g)

4.2 Micropipette: single channel 20 l-200 l, 100 l-1000 l, multi-channel 300 l

4.3 Reagents: methanol, n-hexane, trichloromethane or dichloromethane

5. Sample pre-processing

5.1 Instructions before sample processing:

Apparatus must be clean and disposable suction heads should be used to prevent contamination from interfering with the results of the experiment.

5.2 with liquid:

Liquid 1: sample extract

70% methanol, i.e. V methanol :V deionized water = 7:3.

5.3 Sample pre-treatment steps:

5.3.1 Corn treatment methods

1) Wage 2g of the crushed sample into a 50mL centrifuge tube, add 5mL of sample extract, and oscillate for 5 min at 4000 RPM/separation center at room temperature for 10 min;

2) Take 0.5ml supernatant, add 0.5ml deionized water, and mix;

3) Take 50μl for analysis.

Sample dilution factor: 5 Lower detection limit: 0.15 PPB

5.3.2 Treatment methods for samples with strong water absorption, such as corn husk and wheat bran

1) Wage 2g of the crushed sample into a 50ml centrifuge tube, add 20ml of sample extract, and oscillate for 5 min at 4000 RPM/separation center for 10 min;

2) Take 0.5ml supernatant, add 0.5ml deionized water, and mix;

(For highly toxic samples can be diluted with 35% methanol and then tested.

For example, 0.1ml of the mixture in 2) was added to 0.9ml of 35% methanol for mixing. The dilution factor of the final sample was 200, and the detection limit was 6ppb.)

3) Take 50μl for analysis.

Sample dilution factor: 20 Lower detection limit: 0.6 PPB

Note: Since the distribution of toxin in the sample is non-uniform, it is necessary to take multiple samples and fully mix them before taking 2g for test.

5.3.3 Treatment methods of compound feed

1) Wage 2g of the crushed sample into a 50mL centrifuge tube, add 10mL of sample extract, and oscillate for 5 minutes at 4000 RPM/separation center at room temperature for 10 minutes;

2) Take 0.5ml supernatant, add 0.5ml deionized water, and mix;

3) Take 50μl for analysis.

Sample dilution factor: 10 Lower detection limit: 0.3 PPB

5.3.4 Edible oil, peanut and high fat feed treatment methods

1) Weighed 2g of the crushed sample into a 50mL centrifuge tube, added 8mL of n-hexane and 10mL of sample extract, and oscillated for 5 min at 4000 RPM/separation center at room temperature for 10 min;

2) Remove the upper liquid, take 0.5ml of the lower liquid, add 0.5ml of deionized water, mix, then take 0.5ml of the mixed liquid, add 0.5ml of 35% methanol, and shake for 30S;

3) Take 50μl for analysis.

Sample dilution factor: 20 Lower detection limit: 0.6 PPB

5.3.5 sauces, wheat, biscuits, cakes and other food or seasonings, as well as feed concentrate and other feed processing methods

1) Wage 2g of the crushed sample into a 50mL centrifuge tube, add 10mL of sample extract, and oscillate for 5 minutes at 4000 RPM/separation center at room temperature for 10 minutes;

2) Take 2ml supernatant, add 4ml trichloromethane (or dichloromethane), shake for 5 min, 4000 RPM at room temperature/separation center for 10 min;

3) Transfer the upper liquid to another container, leave the lower liquid for reserve, add another 4ml trichloromethane (or dichloromethane) to the upper liquid, oscillate fully and mix for 5 minutes, at room temperature 4000 RPM/separation center for 10 minutes;

4) Remove the upper liquid, merge the lower liquid twice and fully mix;

5) Take 2ml of the combined lower liquid and dry it under nitrogen at 50-60℃;

6) Add 0.5ml sample extract to fully dissolve the dry matter, and then add 0.5ml deionized water to mix;

7) Take 50μl for analysis.

Sample dilution factor: 10 Lower detection limit: 0.3 PPB

5.3.6 Beer treatment method

1) The beer was fully stirred (CO2 was removed), 2ml of the beer sample was taken, 1ml of distilled water was added, and 7ml of methanol was added, shaking for 5 minutes;

2) Add 0.5 mL of the mixed sample solution into 0.5 mL of deionized water and mix;

3) Take 50μl for analysis.

Sample dilution factor: 10 Lower detection limit: 0.3 PPB

5.3.7 Processing methods of wine, soy sauce and vinegar

1) Take 2ml sample, add 2ml distilled water, then add 10ml trichloromethane (or dichloromethane), oscillate for 5 minutes, 4000 RPM at room temperature/separation center for 10 minutes;

2) Take 1ml of the lower liquid and dry it under nitrogen at 50-60℃;

3) Add 0.5ml sample extract to fully dissolve the dry matter, and then add 0.5ml deionized water to mix;

5) Take 50μl for analysis.

Sample dilution factor: 5 Lower detection limit: 0.15 PPB

6. Procedures for enzyme-linked immunoassay

The reagents needed were taken out of the refrigerated environment at 4℃ and balanced at room temperature for more than 30min. Crystallization may occur in the washing solution during refrigeration and it needs to be restored to room temperature to be fully dissolved. Each liquid reagent must be shaken well before use.

Take out the required number of microplates and frames, put the unused microplates into a self-sealing bag, and store them at 2-8℃.

Before the experiment, dilute 20× concentrated detergent solution with deionized water at 20 times to working detergent solution.

6.1 Numbering: Number the microholes corresponding to the sample and the standard product in sequence, parallel each sample with the standard product by 2 Wells, and record the location of the standard hole and the sample hole.

6.2 Sample adding reaction: add standard or sample 50 l/ well to the respective microwells, then add enzyme marker 50 l/ well, then add 50 l/ well antibody working solution, seal the plate with the cover plate membrane, shake gently for 5 seconds, mix, and react at 25℃ for 30 minutes.

6.3 Washing: Carefully uncover the cover plate membrane, dry the liquid in the hole, fully wash with working washing solution 250 l/ hole for 5 times, each time at an interval of 30 seconds, pat dry with absorbent paper (the air bubbles that have not been removed after pat dry can be pierced with a clean gun).

6.4 Colour development: Add 50 l of substrate solution A and 50 l of substrate solution B to each well, shake gently for 5 seconds and mix well, and develop color at 25℃ for 15 minutes away from light.

6.5 Finishing: Add 50 l terminating solution to each well, shake and mix gently to terminate the reaction.

6.6 Absorbance measurement: Aflatoxin tester was used to measure the Absorbance of each well at 450nm (dual-wavelength 450/630nm is recommended).

The determination should be completed within 10 minutes after the termination of the reaction

6.7 ST-2000A automatic aflatoxin tester automatically complete the determination, automatic results.

Shandong Shengtai Instruments Co., Ltd. is specialized in the research and production of experimental testing instruments and industrial testing instruments. It is a professional instrument company integrating instrument research and development, manufacturing, sales and service. Its products are widely used in food, flour, grain and oil, Feed, electricity, petrochemical, industrial oil, aerospace, coal quality and other industries.

For more than 10 years, Shengtai has always been adhering to the "first-class service, customer first" as the purpose, "reasonable price, honesty and trustworthiness" as the business policy. Adhere to technical innovation, with rich knowledge of the technical team and experienced, thoughtful after-sales service team.

Shengtai instrument as one of the most large-scale and competitive enterprises in the industry, with the strength of technology research and development, professional technical knowledge and innovative design concept, in the model improvement, Significant technical breakthrough has been achieved in the development of function expansion and intelligent control system.

OUR service

After-sales service commitment, our company to sell the product after-sales service commitment: warranty period of one year, long-term technical support outside the warranty period. During the warranty period, the seller shall bear the cost of the replacement of the equipment, the freight and maintenance of the instrument; outside the warranty period, the seller shall waive the maintenance fee and charge only the cost of the basic materials and freight charges when the instrument is malfunctioning and the seller shall not charge the maintenance fee during the warranty period.

Packaging & Delivery

1. Out packing:Standard export wooden case.
2. Inner packing:With careful stretch film wrap product, hard wood board+ strong bandage to fix corners.
3. Checking teams: Specialized staff to inspect and classified your goods.

4.Port Any port of China

Lead Time :

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